Anti-phospho-VASP [pThr²⁷⁸] antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Vasodilator-stimulated phosphoprotein (VASP) is located on human chromosome 19q13.32. It belongs to enabled (Ena) family of proteins and encodes a protein of 46 kDa. VASP has three domain organisation, that is, an N-terminal Ena/VASP (EVH1) homology domain, proline rich central domain and EVH2 actin binding domain.
VASP is localized at highly dynamic membrane regions, focal adhesion sites, lamellipodia protrusions, filopodia tips, and along stress fibers. It is also localized at cell-matrix and cell-cell contacts.

特异性

Anti-phospho-VASP [pThr278] specifically recognizes human VASP phosphorylated at Thr278.

免疫原

synthetic peptide containing phosphorylated Thr²⁷⁸of human VASP, conjugated to KLH. The corresponding sequence is identical in mouse and rat VASP.

应用

Anti-phospho-VASP [pThr278] antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

生化/生理作用

VASP plays an important role in cell motility and adhesion. This protein is also involved in adherens junction formation and stabilization in epithelial cells. VASP is a substrate for cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP)-dependent protein kinases. VASP is required for endothelial barrier functionin vivo. Knockout of VASP proteins in mice leads to increased endothelial permeability causing fatal vascular leakage and hemorrhage during late embryonic development.
Regulation of phosphorylation of Vasodilator-stimulated phosphoprotein (VASP) is observed during actin cytoskeleton formation. It promotes protrusion and propulsion speed and inhibits branching of actins. It plays a key role in the signal transduction pathway related to neutrophil spreading.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~45 kDa
species reactivity	rat, human, mouse
浓度	~0.1 mg/mL
technique(s)	immunoprecipitation (IP): 40 μL using HEK-293T cells overexpressing human VASP western blot: 1:5000-10000 using human platelets cell lysates
UniProt登记号	P50552
运输	dry ice
储存温度	?20℃
Gene Information	human ... VASP(7408) mouse ... Vasp(22323) rat ... Vasp(361517)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable