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位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2

Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2

品牌
Sigma-Aldrich
货号
MABS829
规格纯度
clone 3E7.2, from mouse
价格
5854.74 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2
Epidermal growth factor receptor, EC: 2.7.10.1, Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Epidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1; Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is activated by binding of its specific ligands and transforms from a monomeric to active homodimeric form. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine residues (Y992, Y1045, Y1068, Y1148 and Y1173) occurs in the C-terminal domain. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding domains. EGFR activation results in at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. These downstream events modulate several cell functions, including migration, adhesion, and proliferation. Endocytosis and inhibition of the activated EGFR by phosphatases constitute key regulatory mechanisms.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids surrrounding Tyrosine 1173 of human epidermal growth factor receptor.

应用

Detect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: 10 ?g/mL from a representative lot detected phospho-EGFR (Tyr1173) in A431 cells treated with EGF.

质量

Evaluated by Western Blotting in A431 cells treated with EGF.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 ?g lysate from A431 cells treated with EGF.

目标描述

~200 kDa observed; 134.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 05-483

外形

Protein G purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 3E7.2, monoclonal
species reactivity human
technique(s) immunocytochemistry: suitable
western blot: suitable
同位素/亚型 IgG2bκ
NCBI登记号 NP_005219
UniProt登记号 P00533
运输 ambient

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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