Anti-phospho USP7 (Ser18) Antibody

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一般描述

Ubiquitin-specific-processing protease 7 (USP7) is also known as Ubiquitin carboxyl-terminal hydrolase 7 or Herpesvirus-associated ubiquitin-specific protease (HAUSP). USP7 is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, USP7 activity generally stabilizes its substrate proteins, and it is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.

免疫原

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human USP7 phosphorylated at Ser18.
Epitope: Near N-terminus

应用

Research Sub Category
Apoptosis - Additional
This Anti-phospho USP7 (Ser18) antibody is validated for use in western blotting & peptide inhibition assay for the detection of phospho USP7 (Ser18).
Peptide Inhibition Analysis: 0.2 ?g/mL from a representative lot was blocked by a phospho-peptide in HeLa cell lysate.

Western Blotting Analysis: 0.02 ?g/mL from a representative lot detected phospho USP7 (Ser18) in HeLa cell lysate (Prof. Grigory Dianov, University of Oxford, U.K.).

Western Blotting Analysis: 0.02 ?g/mL from a representative lot detected 100 ng of phospho USP7 (Ser18), and not dephosphorylated USP7 (Prof. Grigory Dianov, University of Oxford, U.K.).

Alexa Fluor^?is a registered trademark of Life Technologies .
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 ?g/mL of this antibody detected phospho USP7 (Ser18) in 10 ?g of HeLa cell lysate.

目标描述

~140 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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