Anti-phospho ULK1 Antibody (Thr574)

半岛bd体育手机客户端 说明

一般描述

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

免疫原

KLH-conjugated linear peptide corresponding to mouse ULK1 phosphorylated at Thr574.

应用

Western Blotting/Peptide Inhibition Analysis: 0.5 ?g/mL from a representative detected phospho ULK1 in WB/PIA.
Detect Autophagy-related protein;ATG1 using this rabbit polyclonal antibody, Anti-phospho ULK1 Antibody (Thr574) validated for use in western blotting & Peptide Inhibition Assay.

质量

Evaluated by Western Blotting in untreated and Calyculin A with okadaic acid treated Jurkat cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected phospho ULK1 (Thr574) in 10 ?g of Jurkat cell lysate.

目标描述

~120 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Affinity purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)	inhibition assay: suitable (peptide) western blot: suitable
NCBI登记号	NP_033495
UniProt登记号	O70405
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable