Anti-phospho PKA-RII (Ser96) Antibody

半岛bd体育手机客户端 说明

一般描述

cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.

特异性

The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.

免疫原

Epitope: Phosphorylated Ser96 of PKA-RIIalpha
KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.

应用

Research Category
Cell Structure

Signaling
Research Sub Category
Cytoskeletal Signaling

Kinases & Phosphatases
This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

质量

Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 ?g/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 ?g of Forskolin untreated and treated PC12 cell lysates.

目标描述

~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.

联系

Replaces: 06-704

外形

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Forskolin untreated and treated PC12 cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat
species reactivity (predicted by homology)	mouse (based on 100% sequence homology)
technique(s)	western blot: suitable
NCBI登记号	NP_032950
UniProt登记号	P12367
运输	wet ice
Gene Information	human ... PRKAR2A(5576)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable