半岛bd体育手机客户端 说明
一般描述
HuR , also known as Hu-antigen R, ELAV-like protein 1, and encoded by the gene name ELAVL1 and HUR, is a member of the RRM ELAV family. HuR is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. Recent studies have shown that HuR is upregulated in certain cancers such as breast and gastric cancers. HuR is also involved in embryonic stem cells (ESCs) differentiation. HuR preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, and thus promoting ESCs differentiation. Additionally, HuR has been shown to bind to poly-U elements and AU-rich elements (AREs) in the 3′-UTR of target mRNAs, as well as the AU-rich element in FOS and IL3/interleukin-3 mRNAs. Some of HuR’s targets include cFos, IL-3, and BRCA1 mRNAs. HuR is also involved in the regulation of VEFG-A and NFkB expression in certain cell types. HuR activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.
免疫原
KLH-conjugated linear peptide corresponding to human phospho HuR (Ser221).
Epitope: Phosphorylated Ser221
应用
Peptide Inhibition Analysis: A 1:200 dilution from a representative lot blocked phospho HuR (Ser221) in HCT116 cell lysate.
Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
Anti-phospho HuR (Ser221) | ABE265 is an antibody against phospho HuR for use in Western Blotting, Peptide Inhibition Assay.
质量
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: A 1:200 dilution of this antibody detected phospho HuR (Ser221) in 10 ?g of HCT116 cell lysate.
目标描述
~37 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | mouse (based on 100% sequence homology),Xenopus(based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), porcine (based on 100% sequence homology) |
technique(s) | inhibition assay: suitable (peptide) western blot: suitable |
NCBI登记号 | NP_001410 |
UniProt登记号 | Q15717 |
运输 | wet ice |
Gene Information | human ... ELAVL1(1994) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich