Anti-phospho Histone H3.1/H3.2 Antibody (Ser87)

半岛bd体育手机客户端 说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3.1, H3.2 and H3.3 are all closely related Histone H3 variants, that despite differing by only a few amino acids have been shown to have different functional roles.

特异性

This antibody recognizes Histone H3.1/H3.2 phosphorylated at Ser87. This antibody demonstrates specificity for phospho Histone H3.1/H3.2 (Ser87), but does not cross react with phospho Histone H3.3, or any other modified and non-modified histones.

免疫原

Epitope: Phosphorylated at Ser87
KLH-conjugated linear peptide corresponding to human Histone H3.1/H3.2 phosphorylated at Ser87.

应用

Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.1/H3.2 Antibody (Ser87) validated for use in western blotting & Dot Blot.
Dot Blot (Specificity) Analysis: 1?g/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in a panel of unmodified and modified Histones.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in Colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in 10 ?g of Colcemid treated HeLa acid extract

目标描述

~17 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa acid extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	bovine, mouse, human
technique(s)	dot blot: suitable western blot: suitable
NCBI登记号	NP_003520
UniProt登记号	P68431
运输	wet ice
Gene Information	human ... SNCA(6622)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable