半岛bd体育手机客户端 说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
免疫原
KLH-conjugated linear peptide corresponding to human Histone H3 (Ser10).
应用
Research Sub Category
Nuclear Receptors
This Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).
Immunocytochemistry Analysis: A 1:5000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cells (Prof. Taro Tachibana, Osaka City University).
ELISA Analysis: A representative lot detected Histone H3 (Ser10) using differently phosphorylated and unphosphorylated peptides unmodified H3, H3 S10ph, and H3 S28ph (Prof. Taro Tachibana, Osaka City University).
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Histone H3 (Ser10) in 10 ?g of nocodazole treated HeLa cell lysates.
目标描述
~17 kDa observed
联系
Replaces: 04-1093
外形
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | rat |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 6G8B7, monoclonal |
species reactivity | human |
浓度 | 1 mg/mL |
technique(s) | ELISA: suitable immunocytochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2aκ |
NCBI登记号 | NP_002098 |
UniProt登记号 | P84243 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich