Anti-phospho Angiomotin Antibody (Ser175)

半岛bd体育手机客户端 说明

一般描述

Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.

特异性

This antibody only detects p130 isoform.

免疫原

Epitope: Phosphorylated Ser175
Linear peptide corresponding to human phospho Angiomotin (Ser175).

应用

Anti-phospho Angiomotin Antibody (Ser175) | ABS1045 is an antibody against phospho Angiomotin Antibody for use in Western Blotting.
Western Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

质量

Evaluated by Western Blotting in HEK293 cell lysate transfected with LATS (Angiomotin Kinase).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho Angiomotin (Ser175) in 10 ?g of HEK293 transfected with LATS.

目标描述

~150 kDa observed. The calculated molecular weight is 118 kDa, however phospho Angiomotin (Ser175) has been shown as a ~150 kDa band in western blots (Yi, C., et al. (2011). Cancer Cell. 19(4):527-540).

外形

T-gel
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)	western blot: suitable
NCBI登记号	NP_001106962
UniProt登记号	Q4VCS5
运输	wet ice
Gene Information	human ... AMOT(154796)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable