Anti-PHF1

半岛bd体育手机客户端 说明

一般描述

Information PHD finger protein 1 (UniProt: O43189; also known as PHF1, hPHF1, Polycomb-like protein 1, hPCl1) is encoded by the PHF1 (also known as PCL1) gene (Gene ID: 5252) in human. PHF1 is a member of the polycomblike family and contains two PHD-type zinc fingers (aa 87-142 and 186-240) and one Tudor domain (aa 29-86). It is essential for epigenetic regulation and genome maintenance. It specifically binds histone H3 trimethylated at ′Lys-36′ (H3K36me3) via its Tudor domain and recruits the PRC2 complex. PHF1 is highly expressed in heart, skeletal muscle, and pancreas, with lower levels found in brain, placenta, lung, liver and kidney. It is involved in DNA damage response and is recruited at double-strand breaks. PHF1 localizes to the promoters of numerous target genes and to double-strand breaks (DSBs) sites following DNA damage. It has been reported that PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. A chromosomal aberration involving PHF1 is reported to be the causative factor in endometrial stromal tumors. (Ref.: Sarma, K et al. (2008). Mol. Cell. Biol. 28(8): 2718-31; Musselman, CA et al (2012). Nat. Struct. Mol. Biol. 19(12):1266-72).

特异性

This rabbit polyclonal antibody detects Information PHD finger protein 1 (PHF1) in human cells. It targets an epitope within 137 amino acids from the internal region.

免疫原

GST-tagged recombinant fraction corresponding to 137 amino acids from the internal region of human PHD finger protein 1.

应用

Anti-PHF1, Cat. No. ABE863, is a highly specific rabbit polyclonal antibody that targets PHD finger protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PHF1 in human pancreas and human kidney tissues.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected PHF1 in 10 ?g of HeLa cell lysate.

质量

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PHF1 in 10 ?g of HeLa cell nuclear extract.

目标描述

~65 kDa observed; 62.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
包装	antibody small pack of 25 μL
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG
NCBI登记号	NP_002627.1
UniProt登记号	O43189
运输	ambient
Gene Information	human ... PHF1(5252)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable