Anti-PERK Antibody, clone 4F6.1

半岛bd体育手机客户端 说明

一般描述

Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1; UniProt Q9NZJ5; also known as Eukaryotic translation initiation factor 2 alpha kinase 3, HsPEK, Pancreatic EIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase) is encoded by the EIF2AK3 (also known as PEK, PERK, WRS) gene (Gene ID 9451) in human. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) induces the activation of three main unfold protein response (UPR) pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) upregulation.

免疫原

Epitope: Internal (C-terminal half)
GST-tagged recombinant protein corresponding to the Internal (C-terminal half) of human PERK.

应用

Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PERK in human liver tissue.
This Anti-PERK Antibody, clone 4F6.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PERK.
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected PERK in 10 ?g of HEK293 cell lysate.

目标描述

~125 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	4F6.1, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_004827
UniProt登记号	Q9NZJ5
运输	wet ice
Gene Information	human ... EIF2AK3(9451)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable