Anti-peptidyl-citrulline, clone F95 Antibody

半岛bd体育手机客户端 说明

一般描述

Deimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.

免疫原

Citrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.

应用

This Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

质量

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.

目标描述

Various kDa calculated

外形

Purified by Ion-Exchange Chromatography
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	F95, monoclonal
species reactivity	human, mouse, rat, chicken
species reactivity (predicted by homology)	all (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgMκ
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable