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Anti-PEPCK

品牌
Sigma-Aldrich
货号
ABC1691
规格纯度
from rabbit, purified by affinity chromatography
价格
5520.38 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-PEPCK
Phosphoenolpyruvate carboxykinase, cytosolic, PEPCK-C
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Phosphoenolpyruvate carboxykinase, cytosolic (UniProt: also known as PEPCK, PEPCK-C) is encoded by the PCK1 (also known as PEPCK1) gene (Gene ID: 5105) in human. PEPCK catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), which is a rate-limiting step in gluconeogenesis. It requires manganese as a cofactor. It is expressed in higher levels in liver of fasting subjects. Kidney and adipocytes exhibit lower expression levels. Two isozymes of PEPCK have been identified, cytosolic and mitochondrial. PEPCK consists of an N-terminal and a catalytic C-terminal domain, with the active site and metal ions located in a cleft between them. Upon binding to substrate, PEPCK undergoes a conformational change that accelerates the catalysis process. Expression of PEPCK can be controlled by cAMP, glucocorticoids, and by insulin. Its expression is induced by glucagon, catecholamines, and glucocorticoids during periods of fasting and in response to stress and is diminished following glucose-induced and by the action of insulin. PEPCK-C is absent in fetal liver but appears at birth, concomitant with the capacity for gluconeogenesis. Deficiency of PEPCK-C is a rare metabolic disorder results in fatty infiltration of liver and kidney, hypoglycemia, hepatomegaly, and failure to thrive.

特异性

This polyclonal antibody detects cytosolic phosphoenolpyruvate carboxykinase in human kidney.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human phosphoenolpyyruvate carboxykinase (PEPCK).

应用

Detect Phosphoenolpyruvate carboxykinase, cytosolic [GTP] using this rabbit polyclonal Anti-PEPCK, Cat. No. ABC1691, tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PEPCK in human kidney, human pancreas, human skeletal muscle, and human placental tissue.

Western Blotting Analysis: 2 ?g/mL from a representative lot detected PEPCK in 10 ?g of human kidney tissue.

Research Category
Apoptosis & Cancer

质量

Evaluated by Immunohistochemistry in human liver tissue

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PEPCK in human liver tissue.

目标描述

~65 kDa observed; 69.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
technique(s) immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号 NP_002582
UniProt登记号 P35558
运输 ambient
Gene Information human ... PCK1(5105)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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