Anti-PBP2a of MRSA antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Infections caused by methicillin-resistantStaphylococcus aureus(MRSA) are a major concern cause for clinicians, presenting high mortality and morbidity rates compared to infections caused by methicillin-sensitiveStaphylococci.2

特异性

Monoclonal Anti-PBP2a of MRSA antibody specifically recognizes PBP2a protein from Methicillin-resistantStaphylococcus aureus(MRSA). The antibody reacts with both denaturated recombinant PBP2a protein and bacteria lysates in Immunoblot.1 The antibody recognizes PBP2a in MRSA cells in both the exponential and stationary phases, however no cross reaction with methicillin-sensitiveStaphylococcus aureus(PBP2a negative) andE. coliwere seen.1

应用

The antibody may be used in various immunochemical techniques including Immunoblotting1 (~76 kDa), ELISA1, Surface Plasmon Resonance (SPR)1 and Flow cytometry1.

生化/生理作用

Resistance ofS. aureusto methicillin and all beta-lactam antibiotics is due to the activity of PBP2a protein (i.e., penicillin binding protein2a) that is located in the cell wall of the resistant strains.3-4 PBP2a is encoded by mecA gene and belongs to a family of penicillin binding proteins (PBPs). PBPs are membrane-bound enzymes that catalyze the transpeptidation reaction necessary for cross-linkage of peptidoglycan chains.5-6 Contrary to all PBPs, PBP2a has low affinity for all ?-lactam antibiotics.7 Therefore, in the presence of ?-lactam antibiotics, while normal PBPs are blocked, the PBP2a is able to continue catalyze the transpeptidation reactions enabling cell wall synthesis.4 PBP2a protein is suggested to be a promising candidate for anti-MRSA vaccine development.8

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.Antibody Concentration: ~ 1.0 mg/mL

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	38, monoclonal
形式	liquid
浓度	~1 mg/mL
technique(s)	immunoblotting: 0.5-1 μg/mL using whole recombinant PBP2a protein
同位素/亚型	IgG1
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable