Anti-Pan DIDO Antibody

半岛bd体育手机客户端 说明

一般描述

Death-inducer obliterator 1 (UniProt Q9BTC0; also known as DATF-1, Death-associated transcription factor 1, DIO-1, hDido1) is encoded by the DIDO1 (also known as C20orf158, DATF1, KIAA0333) gene (Gene ID 11083) in human. The DIDO1 gene encodes four alternatively spliced variants (DIDO1/isoform 2, DIDO2/isoform 1, DIDO3/isoform 4, isoform a/isoform 3) implicated to be involved in apoptosis and development. The longest and most widely expressed DIDO3 is assigned as the canonical isoform. DIDO3 is a nuclear protein that forms part of the spindle assembly checkpoint (SAC) and is necessary for correct chromosome segregation in somatic and germ cells. Dido3-knockout compromises differentiation of murine embryonic stem cells (mESCs) in vitro and of epiblast cells in vivo, resulting in early embryonic death at around day 8.5 of gestation. On the other hand, exogenous expression of Dido1 isoform in mESCs is reported to inhibit LIF withdrawal-induced differentiation, while Dido1 knockdown facilitate mESCs differentiation.

特异性

This polyclonal antibody detects an N-terminal epitope present in all four spliced isoforms of human DIDO1 reported by UniProt (Q9BTC0).

免疫原

KLH-conjugated linear peptide corresponding an N-terminal sequence of human DIDO1.

应用

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DIDO immunoreactivity in human kindey, human prostate cancer and human cerebellum tissue.
This Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO.

质量

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected DIDO isoforms in 10 ?g of HepG2 cell lysate.

目标描述

~260/130/79/70/66 observed. 59.46 kDa (isoform 2; DIDO1), 129.2 kDa (isoform 1; DIDO2), 243.9 kDa (isoform 4; DIDO3), 61.35 kDa (isoform 3; isoform a) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Affinity purified.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	nonhuman primates (based on 100% sequence homology),Xenopus(based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_071388
UniProt登记号	Q9BTC0
Gene Information	human ... DIDO1(11083)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable