Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).

免疫原

recombinant, human p53 expressed inE. coli
Epitope: within amino acids 37-45 of p53

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)

警告

Toxicity: Standard Handling (A)

分析说明

Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue

其他说明

Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Lane, D.P. 1992.Nature358,15.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	DO-7, monoclonal
形式	liquid
不包含	preservative
species reactivity	bovine, monkey, human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG2b
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable