Anti-NRF-1 Antibody, clone R157.1.3H3

半岛bd体育手机客户端 说明

一般描述

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

免疫原

Recombinant protein corresponding to human NRF-1.

应用

Research Sub Category
Nuclear Receptors
This Anti-NRF-1 Antibody, clone R157.1.3H3 is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of NRF-1.
Western Blotting Analysis: 1.0 ?g/mL from a representative lot detected NRF-1 in 10 ?g of SH-SY5Y and HeLa cell lysates.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NRF-1 chromatin DNA binding activity using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
ChIP-seq Analysis: A representative lot detected NRF-1 DNA binding motifs by ChIP-seq analysis using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected NRF-1 in 10 ?g of Raji cell lysate.

目标描述

~58 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	R157.1.3H3, monoclonal
species reactivity	human
technique(s)	ChIP: suitable (ChIP-seq) western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_001035199
UniProt登记号	Q16656
运输	wet ice
Gene Information	human ... NRF1(4899)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable