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Anti-NPC2 Antibody, clone 13G4

品牌
Sigma-Aldrich
货号
MABS440
规格纯度
clone 13G4, from mouse
价格
5520.38 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-NPC2 Antibody, clone 13G4
Epididymal secretory protein E1, NPC2, Human epididymis-specific protein 1, He1, Niemann-Pick disease type C2 protein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Epididymal secretory protein E1 (UniProt P61916; also known as Human epididymis-specific protein 1, He1, Niemann-Pick disease type C2 protein) is encoded by the NPC2 (also known as HE1) gene (Gene ID 10577) in human. Niemann-Pick C1 & C2 (NPC1 & NPC2) proteins are involved in the transport of cholesterol and other lipids out of late endosomes and lysosomes. NPC1 is a large membrane spanning glycoprotein mainly located in the late endosomes, while NPC2 is a small soluble protein that binds nonesterified cholesterol with high affinity. NPC1 and NPC2 defects affect the intracellular trafficking of unesterified cholesterol and other lipids, causing the rare autosomal recessive lysosomal storage disorder known as Niemann-Pick type C disease. The majority of NPC patients have a defect in NPC1 (95%), while only 5% of the NPC cases are due to mutations in NPC2.

特异性

Clone 13G4 detects Niemann-Pick disease type C2 protein (NPC2). Reactivity toward the spliced isoform 2 (UniProt P61916-2) has not been determined.

免疫原

GST-tagged recombinant human NPC2.

应用

Anti-NPC2 Antibody, clone 13G4 is an antibody against NPC2 for use in Western Blotting.
Western Blotting Analysis: A representative lot detected the intracellular organelle protein NPC2 in whole cell lysates, but not in plasma membrane extracts, from SV-589 human skin fibroblasts (Das, A., et al. (2013). Proc. Natl. Acad. Sci. U. S. A. 110(26):10580-10585).
Research Sub Category
Vesicular Trafficking
Research Category
Signaling

质量

Evaluated by Western Blotting in HT1080 human fibrosarcoma cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected NPC2 in 10 ?g of HT1080 human fibrosarcoma cell lysate.

目标描述

~17 kDa observed. 16.57 kDa (isoform 1) and 13.42 kDa (isoform 2) calculated. A broad, smeared banding pattern in the range from ~17 to 25 kDa is typically seen.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 13G4, monoclonal
species reactivity human
technique(s) western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_006423.1
UniProt登记号 P61916
运输 wet ice
Gene Information human ... NPC2(10577)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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