Anti-NMDAR1 Antibody, clone 54.1

半岛bd体育手机客户端 说明

一般描述

Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.

特异性

Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.

免疫原

Epitope: Extracellular loop between transmembrane regions III & IV.
Recombinant fusion protein containing GST and NMDAR1 aa. 660-811.

应用

Anti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.
Research Category
Neuroscience
Immunocytochemistry:
A previous lot of this antibody was used in IC.

RIA:
A previous lot of this antibody was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA.

Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.

Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.

Optimal working dilutions must be determined by end user.
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by immunohistochemistry on rat brain and spinal cord tissue.

Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord

目标描述

103-116 kDa

外形

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.

储存及稳定性

Stable for 1 year at -20?C from date of receipt.

分析说明

Control
Rat brain and spinal cord tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	54.1, monoclonal
species reactivity	Xenopus, rat, human, monkey
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable radioimmunoassay: suitable
同位素/亚型	IgG2a
NCBI登记号	NM_000832.5
UniProt登记号	Q05586
运输	dry ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable