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位置: 首页> 品牌> Sigma-Aldrich> Anti-Neutrophil Elastase Antibody, clone AHN-10

Anti-Neutrophil Elastase Antibody, clone AHN-10

品牌
Sigma-Aldrich
货号
MABS461
规格纯度
clone AHN-10, from mouse
价格
5520.38 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Neutrophil Elastase Antibody, clone AHN-10
Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Neutrophil Elastase, also known as Bone marrow serine protease, Elastase-2, Human leukocyte elastase (HLE), Medullasin or PMN elastase, and encoded by the gene ELANE/ELA2, is a serine proteinase in the same family as chymotrypsin. Neutrophil Elastase has broad substrate specificity and is part of our host defense mechanism against bacteria. Unfortunately passively released or actively secreted elastase from neutrophils has been linked to the pathologic processes of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome, and cystic fibrosis. Neutrophil Elastase is localized to the cytoplasm in granules. Neutrophil Elastase is expressed in neutrophils and macrophages. Defects in Neutrophil Elastase may be associated with cyclic neutropenia and congential neutropenia diseases where neutrophils fail to mature leaving the patient very vunderable to infections.

特异性

This antibody detects with human Neutrophil Elastase but not other major neutrophil proteases: Cathapsin G, proteinase 3, collagenase, or esterase N (Skubitz, K. M., et al. (1988). J Leukoc Biol. 44(3):158-165.).

免疫原

Whole cells corresponding to human Neutrophil Elastase.

应用

Research Category
Cell Structure
Detect Elastase using this mouse monoclonal antibody, Anti-Neutrophil Elastase Antibody, clone AHN-10 validated for use in ICC, IHC, IP, Radioimmunoassay & Inhibition.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in unirradiated and irradiated human and mouse skin tissues (Rhodes, L. E., et al. (2009). FASEB J. 23(11): 3947-3956.; Nicolaou, A., et al. (2012). Photochem Photobiol Sci. 11(2):371-380.; Rutter, J. J., et al. (2009). 129(12):2861-2867.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human acinar cells (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).

Inhibition Analysis: This assay inhibits the elastinolytic actiity of purified human Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.).

Radioimmunoassay Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase using purified Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.).
Research Sub Category
ECM Proteins

质量

Evaluated by Immunocytochemistry Analysis in peripheral blood mononuclear cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Neutrophil Elastase in peripheral blood mononuclear cells.

目标描述

28 kDa calculated

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 AHN-10, monoclonal
species reactivity human
technique(s) immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
radioimmunoassay: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_001963.1
UniProt登记号 P08246
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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