Anti-Neurocan Antibody, clone 650.24

半岛bd体育手机客户端 说明

一般描述

Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.

特异性

Neurocan. The specificity of clone 650.24 was checked by western blots of brain lysates and purified neurocan; the antibody reacted with the identical bands that clones 1D1 and 1F6 (other neurocan monoclonals) reacted; furthermore, the staining pattern of brain sections with these antibodies were all identical. Finally 650.24 also recognized recombinant neurocan expressed in transfected 293 cells by western blot.

免疫原

Embryonic rat brain proteoglycans

应用

Western blot: 1-2 μg/mL. Reacts with polypeptides of 260 and 160 kDa on western blots of embryonic rat brain tissue extracts. The 160 kDa species is typically only seen aftyer chondroitinase treatment.Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

Immunocytochemistry: 1:5

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000

Immunoprecipitation: 1-2 μg/mL

Optimal working dilutions must be determined by the end user.
This Anti-Neurocan Antibody, clone 650.24 is validated for use in IP, WB, IC, IH for the detection of Neurocan.
Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine

Growth Cones & Axon Guidance

外形

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

分析说明

Control
POSITIVE CONTROL:

early post-natal rat brain. Not expressed in kidney, lung, liver, or muscle.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	650.24, monoclonal
species reactivity	rat
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_004386.2
UniProt登记号	O14594
运输	wet ice
Gene Information	human ... NCAN(1463)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable