半岛bd体育手机客户端 说明
一般描述
Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.
特异性
Neurocan, chicken. Recognizes the middle region of neurocan.
应用
Anti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB.
Western blot: <0.5 μg/mL
Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunoprecipitation: 1 μg/mL
ELISA: 1 μg/mL, excellent for core protein, good for monomer
Immunocytochemistry: not tested
Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.
Optimal working dilutions must be determined by the end user.
外形
Format: Purified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | monoclonal |
species reactivity | chicken |
manufacturer/tradename | Chemicon? |
technique(s) | ELISA: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_004386.2 |
UniProt登记号 | O14594 |
运输 | dry ice |
Gene Information | human ... NCAN(1463) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich