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位置: 首页> 品牌> Sigma-Aldrich> Anti-Neurocan Antibody

Anti-Neurocan Antibody

品牌
Sigma-Aldrich
货号
MAB5234
规格纯度
Chemicon?, from mouse
价格
5854.74 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Neurocan Antibody
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.

特异性

Neurocan, chicken. Recognizes the middle region of neurocan.

应用

Anti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB.
Western blot: <0.5 μg/mL

Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.

Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

Immunoprecipitation: 1 μg/mL

ELISA: 1 μg/mL, excellent for core protein, good for monomer

Immunocytochemistry: not tested

Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.

Optimal working dilutions must be determined by the end user.

外形

Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 monoclonal
species reactivity chicken
manufacturer/tradename Chemicon?
technique(s) ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1
NCBI登记号 NM_004386.2
UniProt登记号 O14594
运输 dry ice
Gene Information human ... NCAN(1463)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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