Anti-Neurexin-1-alpha Antibody

半岛bd体育手机客户端 说明

一般描述

Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion (see review by Missler & Sudhof, 1998). Antibody Anti-Neurexin-1-alpha (ABN35) was made against a peptide immunogen corresponding to a stretch of amino acids in the extracellular domain of human Neurexin-1-alpha (Uniprot Q9ULB1). Although the theoretical full length of Neurexin-1-alpha is 161 kDa, the protein detected in human brain lysates may show a band around 160 kDa and additional bands around 45 kDa as a possible result of the posttranslational processing previously reported.

特异性

This antibody recognizes Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

免疫原

Epitope: Extracellular laminin G-like 3 domain
KLH-conjugated Linear peptide corresponding to human Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

应用

Detect Neurexin-1-alpha using this Anti-Neurexin-1-alpha Antibody validated for use in WB, IH(P).
Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-alpha in human brain tissue.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blot in human cerebellum tissue lysate.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected Neurexin-1-alpha on 10 ?g of human cerebellum tissue lysate.

目标描述

~ 45 kDa Observed At higher concentrations or longer exposures, in certain lysates, a band at ~150kD can be seen that corresponds to the expected full length Mw.
UniProtKB/Swiss-Prot Q9ULB1 (NRX1A_HUMAN) entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. Reference (Accolade/Related Documents) TIG JANUARY 1998 VOL. 14 No. 1 indicates that multiple neurexin isoforms are generated from three genes by alternative promoters and alternative splicing.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human cerebellum tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human, rat
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_004792
UniProt登记号	Q9ULB1
运输	wet ice
Gene Information	human ... NRXN1(9378)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable