Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324

半岛bd体育手机客户端 说明

一般描述

Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts

特异性

Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.

免疫原

Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.

应用

This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.

Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.

Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

Optimal working dilutions must be determined by end user.

目标描述

120-140 kDa

外形

Protein A purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	324, monoclonal
species reactivity	mouse, rat
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG
NCBI登记号	NP_032504.3
UniProt登记号	P11627
运输	wet ice
Gene Information	human ... L1CAM(3897), VCAM1(7412)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2