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Anti-NEDD9 Antibody

品牌
Sigma-Aldrich
货号
ABT166
规格纯度
from rabbit, purified by affinity chromatography
价格
4592.35 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-NEDD9 Antibody
Enhancer of filamentation 1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12 p105
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Enhancer of filamentation 1, also known as CRK-associated substrate-related protein (CAS-L) or Cas scaffolding protein family member 2 or Neural precursor cell expressed developmentally down-regulated protein 9 (NEDD-9), or Renal carcinoma antigen NY-REN-12 p105, and encoded by the gene NEDD9 or CASL or CASS2, is a docking protein that plays a central role in coordinating tyrosine kinase mediated signaling for cell adhesion. NEDD9 controls the signals between focal adhesions at the cell periphery and the mitotic spindle in response to growth factors and signals. NEDD9 interacts with integrins and various other cellular proteins including BCAR3 and SH2D3C. It is localized to the cytoplasm in most cells but location can vary depending upon the cell type and function. NEDD9 is also found in cellular projections and lamellipodium and is found in the nucleus depending upon the cellular microenvironment. NEDD9 has multiple isoforms and active proteolytic fragments ranging from 55 kDa to 115 kDa. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle. NEDD9 is highly expressed in most cells and tissues and is typically induced upon cell growth and down regulated in adult tissues such as brain. NEDD9 is processed during the cell cycle into multiple proteolytic fragments that appear to have important functions at specific times during the cell cycle process. In cancer NEDD9 over expression can promote tumor growth and NEDD9 acts directly to stabilize Aurora A kinase which is often over expressed in many cancers. Destabilization of Aurora A via the inhibition or destruction of NEDD9 allows for tumor reduction, thus NEDD9 expression is being examined as a potential cancer marker.

免疫原

KLH-conjugated linear peptide corresponding to human near the C-terminus of NEDD9.
Epitope: Near C-terminus

应用

Research Category
Cell Structure
Detect Nedd-9 using this rabbit polyclonal antibody, Anti-NEDD9 Antibody validated for use in western blotting.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NEDD9 in 10 ?g of HUVEC cell lysate.

目标描述

~110 kDa observed. The calculated molecular weight of this protein is 92 kDa, but can be observed at ~105-115 kDa due to phosphorylation. (Bradbury, P., et al. (2012). BBA. 290-297). A cleavage form of this prortein may be observed at ~55 kDa (Law, S., et al. (1998). Molecular Cellular Biology. 18(6):3540-3551). Uncharacterized band(s) may appear in some lysates.

外形

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity mouse, human, rat
species reactivity (predicted by homology) bovine (based on 100% sequence homology)
technique(s) western blot: suitable
NCBI登记号 NP_006394
UniProt登记号 Q14511
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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