Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9

半岛bd体育手机客户端 说明

一般描述

Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.

特异性

Na+/H+ exchanger, isoform NHE1

免疫原

MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.
Epitope: C-terminus

应用

Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.

Immunoblotting:
1:1,000 dilution used on a previous lot.

Optimal working dilutions must be determined by the user.

质量

Routinely evaluated by Western Blot on Human Kidney lysates.

Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.

目标描述

~100-110 kDa

外形

Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at 2-8?C from date of receipt.

分析说明

Control
Kidney tissue (basolateral membrane)

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	4E9, monoclonal
species reactivity	amphibian, vertebrates (including including fish, amphibians, birds and mammals), avian, fish
species reactivity (predicted by homology)	mammals, mouse, rat
manufacturer/tradename	Chemicon?
technique(s)	western blot: suitable
同位素/亚型	IgG1
适用性	not suitable for immunoprecipitation
NCBI登记号	NM_003047.2
UniProt登记号	P19634
运输	wet ice
Gene Information	human ... SLC9A1(6548)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable