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位置: 首页> 品牌> Sigma-Aldrich> Anti-N-cadherin Antibody, clone 6A9.2

Anti-N-cadherin Antibody, clone 6A9.2

品牌
Sigma-Aldrich
货号
MABT530
规格纯度
clone 6A9.2, from mouse
价格
5413.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-N-cadherin Antibody, clone 6A9.2
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Cadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. N-Cadherin is a single-pass type I membrane protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. Cadherins are synthesized as precursor proteins that are proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis.

特异性

Clone 6A9.2 targets an epitope within N-cadherin extracellular cadherin 1 domain.

免疫原

GST/His-tagged recombinant human N-cadherin extracellular cadherin 1 domain fragment.

应用

Research Category
Cell Structure
This mouse monoclonal Anti-N-cadherin antibody, clone 6A9.2, Cat. No. MABT530 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of N-cadherin.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected N-cadherin in human cervical cancer, adrenal gland, and liver tissue sections.

质量

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected N-cadherin in 10 ?g of C6 rat glioma cell lysate.

目标描述

~140 kDa observed. Target band size appears larger than the calculated molecular weights of 99.81/97.23/82.03 kDa (prepro-/pro-/mature human N-cadherin) and 99.69/97.24/81.91 kDa (prepro-/pro-/mature rat N-cadherin) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 04-1126

外形

Protein G purified.
Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 6A9.2, monoclonal
species reactivity human, rat
technique(s) immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型 IgG2aκ
NCBI登记号 NP_001783
UniProt登记号 P19022
运输 ambient
Gene Information human ... CDH2(1000)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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