Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1

半岛bd体育手机客户端 说明

特异性

Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.

免疫原

Epitope: C-terminus
Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.

应用

Detect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB.
Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins
Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.

Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.

Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.

Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience

目标描述

23-25 kDa

联系

Replaces: MAB388

外形

Format: Purified
Protein A purified
Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Brain

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	PLPC1, monoclonal
species reactivity (predicted by homology)	mammals
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG2a
NCBI登记号	NM_000533.3
UniProt登记号	P60201
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable