您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-MUTYH Antibody, clone 12D5.1

Anti-MUTYH Antibody, clone 12D5.1

品牌
Sigma-Aldrich
货号
MABC954
规格纯度
clone 12D5.1, from mouse
价格
5358.91 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-MUTYH Antibody, clone 12D5.1
A/G-specific adenine DNA glycosylase, MutY homolog, hMYH
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

A/G-specific adenine DNA glycosylase (UniProt Q9UIF7; also known as hMYH, MutY homolog) is encoded by the MUTYH (also known as MYH) gene (Gene ID 4595) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. MUTYH/MYH is a monofunctional DNA glycosylase that excises mismatched adenine opposite 7, 8-dihydro-8-oxoguanine (8-oxoG), which is the major mutagenic lesion induced by oxidative stress. Biallelic mutations in MUTYH are associated with MUTYH-Associated polyposis (MAP) and increased risk in colorectal cancer (CRC).

特异性

Expected to react with all six spliced isoforms of human MUTYH reported by UniProt (Q9UIF7).

免疫原

GST-tagged recombinant internal fragment from the N-terminal half of human MUTYH.
Epitope: N-terminal half.

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Apoptosis & Cancer
Anti-MUTYH Antibody, clone 12D5.1 is an antibody against MUTYH for use in Western Blotting.

质量

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected MUTYH in 10 ?g of Raji cell lysate.

目标描述

~59 kDa observed. 60.07 kDa (isoform Alpha-1), 59.14 kDa (isoform Alpha-2), 59.07 kDa (isoform Alpha-3), 58.44 kDa (isoform Beta-1), 57.52 kDa (isoform Gamma-2), and 50.52 kDa (isoform Gamma-3) calculated.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 12D5.1, monoclonal
species reactivity human
technique(s) western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_036354
UniProt登记号 Q9UIF7
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map