Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3

半岛bd体育手机客户端 说明

特异性

m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.

SPECIES REACTIVITIES:

Expected to react with most mammalian species.

免疫原

i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.

应用

Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells

Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.

外形

Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Format: Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	M2-2-B3, monoclonal
species reactivity	human, rat, monkey
species reactivity (predicted by homology)	mammals
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable
同位素/亚型	IgG2a
适用性	not suitable for immunohistochemistry (Paraffin)
NCBI登记号	NM_000739.2 NM_001006626.1 NM_001006627.1 NM_001006628.1 NM_001006629.1 NM_001006630.1 NM_001006631.1 NM_001006632.1
UniProt登记号	P08172
运输	wet ice
Gene Information	human ... CHRM2(1129)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable