Anti-Mucin 5B Antibody, clone 19.4E

半岛bd体育手机客户端 说明

一般描述

Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 10³kDa.

特异性

Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.

免疫原

Un-glycosylated, serine- and threonine-free synthetic peptide from the N-terminus of human MUC5B.

应用

Research Sub Category
Tumor Markers
Western blot; Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins.

Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer
Detect Mucin 5B using this Anti-Mucin 5B Antibody, clone 19.4E validated for use in ELISA, WB, IH.

外形

Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.
Format: Purified

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	19.4E, monoclonal
species reactivity	human, monkey
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_002458.1
UniProt登记号	Q9HC84
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable