Anti-MRP1 Antibody, clone QCRL-1

半岛bd体育手机客户端 说明

特异性

Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane

phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines

and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,

MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.

免疫原

Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).

应用

Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).

Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).

Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).

Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).

Immunoprecipitation (Hipfner, et al., 1994; 1996).

Optimal working dilutions must be determined by the end user.

外形

Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
Format: Purified

储存及稳定性

Store at 2-8?C for up to 3 months. For longer term storage, aliquot and freeze at -20?C.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	300
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	QCRL-1, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_004996.3 NM_019862.2 NM_019898.2 NM_019899.2 NM_019900.2 NM_019901.1 NM_019902.1
UniProt登记号	P33527
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable