Anti-Mi-2β Antibody, clone 17H11

半岛bd体育手机客户端 说明

一般描述

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

特异性

Recognizes Mi-2β (CHD-4)

免疫原

Recombinant protein corresponding to Human Mi-2β (CHD-4).

应用

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.

ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.
Research Sub Category
Chromatin Biology

Histone Modifying Proteins
This Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in a Jurkat cell lystate.

Western Blotting Analysis: A 1 ?g/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.

目标描述

~ 220 kDa observed

外形

Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	17H11, monoclonal
纯化方式	affinity chromatography
species reactivity	human
technique(s)	ChIP: suitable (ChIP-seq) western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_001264
UniProt登记号	Q14839
运输	wet ice
Gene Information	human ... CHD4(1108)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable