Anti-MDM2 (Ab-2) Mouse mAb (2A10)

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一般描述

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells.
Purified mouse monoclonal antibody (see application references). Recognizes the ~90 kDa (apparent MW) MDM2 protein.
This Anti-MDM2 (Ab-2) Mouse mAb (2A10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-2).

免疫原

human MDM2 protein
Human
Epitope: within amino acids 294-339

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (2 g/ml)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction, see application references)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
A549 cells or most tissues

其他说明

Antibody should be titrated for optimal results in individual systems.
Marchetti, A., et al. 1995.J. Pathol.175,31.
Barak, Y., et al. 1993.EMBO. J.12,461.
Chen, J., et al. 1993.Mol. Cell Biol.13,4107.
Ladanyi, M., et al. 1993.Cancer Res.53,16.
Leach, F.S., et al. 1993.Cancer Res.53,2231.
Oliner, J.D., et al. 1993.Nature362,857.
Momand, J., et al. 1992.Cell69,1237.
Oliner, J.D., et al. 1992.Nature358,80.
Fakharzadeh, S.S., et al. 1991.EMBO. J.10,1565.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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