Anti-MBD4 antibody produced in rabbit

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一般描述

MBD4 (methyl-CpG binding domain 4, DNA glycosylase) is a methyl-CpG-binding endonuclease. It is a tripartite with an N-terminal 5-methylcytosine binding domain (MBD), a C-terminal catalytic domain with homology to bacterial DNA damage-specific glycosylases/lyases and a core region with five putative nuclear localization signals.

免疫原

Methyl-CpG-binding domain protein 4 recombinant protein epitope signature tag (PrEST)

应用

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

生化/生理作用

MBD4 (methyl-CpG binding domain 4, DNA glycosylase) is involved in the maintenance of genomic integrity. It acts as a DNA repair protein in the DNA mismatch repair (MMR) system. During DNA repair, MBD4 couples with MMR protein MLH1 (mutL homolog 1) that binds to the methyl-CpG-containing DNA to perform endonuclease activity. MBD4 also has impact on glycosylase/lyase activity in a pathway of base excision repair. It has been reported that mutation in MED1 activity may cause irregular DNA damage and tumor development.

特点和优势

Prestige Antibodies^?are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^?to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

联系

Corresponding Antigen APREST86363.

外形

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

法律信息

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

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安全信息