Anti-MAGI3 antibody produced in rabbit

半岛bd体育手机客户端 说明

免疫原

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 (Membrane-associated guanylate kinase inverted 3) (MAGI-3)

应用

Anti-MAGI3 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org). Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

生化/生理作用

MAGI3 (membrane associated guanylate kinase, WW and PDZ domain containing 3) gene encodes a membrane-associated guanylate kinase that contains multi-PDZ domains. It is a scaffolding protein that is found at cell-cell junctions. It interacts with frizzled-4 and -7 (seven-transmembrane proteins involved in PCP/ planar cell polarity pathway) and Ltap (a mouse homolog of theDrosophilaPCP protein, stbm) to form a complex. This complex is found to activate JNK signaling cascade. It binds to the (PDZ) recognition motif (TVV) that is present at the C-terminal of the transforming growth factor-α precursor (proTGFα) and facilitates efficient trafficking of TGFα to the cell surface in polarized epithelial cells. MAGI3 binds to the PDZ domain-binding site of PTEN/MMAC, a tumor suppressor, and modulates the kinase activity of AKT/PKB (protein kinase B). It binds to the PDZ-binding domain of LPA2 (lysophosphatidic acid 2) and regulates the ability of LPA2 to activate Erk (extracellular signal regulated kinase) and RhoA.

特点和优势

Prestige Antibodies^?are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^?to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

联系

Corresponding Antigen APREST71112.

外形

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

法律信息

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

基本信息

人类蛋白质图谱编号

HPA007923 Human Protein Atlas characterization data

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
半岛bd体育手机客户端 线	Prestige Antibodies?Powered by Atlas Antibodies
形式	buffered aqueous glycerol solution
species reactivity	human
包装	antibody small pack of 25 μL
technique(s)	immunohistochemistry: 1:20- 1:50
免疫原序列	LKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRG
UniProt登记号	Q5TCQ9
运输	wet ice
储存温度	?20℃
Gene Information	human ... MAGI3(260425)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)