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位置: 首页> 品牌> Sigma-Aldrich> Anti-Mad2 Antibody, clone AS55-A12

Anti-Mad2 Antibody, clone AS55-A12

品牌
Sigma-Aldrich
货号
MABE866
规格纯度
clone AS55-A12, from mouse
价格
5016.79 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Mad2 Antibody, clone AS55-A12
Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Mad2, also known as MAD2, MAD2L1, or Mitotic spindle assembly checkpoint protein MAD2A, is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. Mad2 then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately from cell death or disease.

免疫原

His-tagged recombinant protein corresponding to human Mad2.

应用

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cell lysate (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad2 in PtK1 cells transfected with Alexa-Mad2 (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cells transfected with pCMV expressing wild-type myc-tagged Mad2, and certain mutations of myc-tagged Mad2 (This mutant does not bind to the core of Mad1/Mad2) (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).
Anti-Mad2 Antibody, clone AS55-A12 is a highly specific mouse monoclonal antibody, that targets MAD2 & has been tested in western blotting & Immunofluorescence.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 ?g/mL of this antibody detected Mad2 in 10 ?g of HEK293 cell lyate.

目标描述

~23 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 AS55-A12, monoclonal
species reactivity human
technique(s) immunofluorescence: suitable
western blot: suitable
同位素/亚型 IgG2b
NCBI登记号 NP_002349
UniProt登记号 Q13257
运输 wet ice
Gene Information human ... MAD2L1(4085)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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