Anti-Liver receptor homolog-1 Antibody, clone 1LRH-2E1

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一般描述

Liver receptor homolog 1 (LRH-1, CPF, FTF, PHR-1, FTF, or hB1F) is an orphan nuclear receptor that is highly expressed in the intestines, pancreas, ovary, and liver. LRH-1 is a constitutively active transcription factor with a complex role in many cellular processes which may include lipid metabolism, cell proliferation, development, reproduction, and immunity. LRH-1 may interact with an array of transcriptional coactivators that modulate its activity, among which are the MBF1, SRC, and PGC-1α proteins. In addition, ERK phosphorylation on Ser-238 and Ser-243 also enhances LRH-1 activity. However, LRH-1 is also negatively regulated by several factors. For instance, corepressors such as SHP, DAX-1, and PROX-1, preclude its binding to DNA; and sumoylation promotes its inactivation and sequestration into nuclear bodies. LRH-1 targets many genes including the CYP7A1 and CYP8B1 genes involved in bile synthesis; and Oct4 required for pluripotency in embryonic stem cells. LRH-1 has been implicated in inflammatory bowel disease, and tumor development in the intestines and pancreas.

免疫原

Linear peptide corresponding to the mouse Liver receptor homolog-1.

应用

Use Anti-LRH-1 Antibody, clone 1LRH-2E1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect LRH-1 also known as Liver receptor homolog 1.
Western Blot Analysis: A 1:1,000 dilution from representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cell lysates (M. Oulad, IGBMC, France.).

Immunocytochemistry Analysis: A representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cells (M. Oulad, IGBMC, France.).

质量

Evaluated by Western Blot in mouse pancreas tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Liver receptor homolog-1 in 10 µg of mouse pancrease tissue lysate.

目标描述

~64 kDa, ~56 kDa, and ~42 kDa observed. This antibody detects three isoforms of Liver receptor homolog-1 as described by Uniprot No. O00482.

外形

Mouse monoclonal IgG2aλ ascites with 0.05% sodium azide.
Unpurified

分析说明

Control
Mouse pancreas tissue lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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