Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1

半岛bd体育手机客户端 说明

一般描述

The protein named Leukemia-associated RhoGEF (LARG) or alternatively known as Leukemia-associated RhoGEF protein, and encoded by the gene ARHGEF12/KIAA0382/LARG, plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) proteins. Leukemia-associated RhoGEF (LARG) acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Additionally, Leukemia-associated RhoGEF (LARG) interacts with RHOA, PLXNB1 and PLXNB2, and it interacts with IGF1R beta subunit and GCSAM proteins. Leukemia-associated RhoGEF (LARG) is localized to the cytoplasm and membrane. The protein translocates to the membrane upon stimulation. Leukemia-associated RhoGEF (LARG) widely expressed with isoform specific isoforms being found in certain tissues only, such as isoform 2 is primarily found in the intestine and testis.

免疫原

Epitope: RGSL Domain
GST-tagged recombinant protein corresponding to the RGSL domain of human Leukemia-associated RhoGEF/LARG.

应用

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Leukemia-associated RhoGEF/LARG in HepG2 and HeLa cells.
Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1 is a highly specific mouse monoclonal antibody, that targets Leukemia-associated RhoGEF & has been tested in western blotting & ICC.

质量

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Leukemia-associated RhoGEF/LARG in 200 ?g of HUVEC cell lysate.

目标描述

~ 173 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	13D2.1, monoclonal
species reactivity	human
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_056128
UniProt登记号	Q9NZN5
运输	wet ice
Gene Information	human ... ARHGEF12(23365)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable