Anti-L1CAM Antibody

半岛bd体育手机客户端 说明

一般描述

Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules (N-CAM), myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts.

特异性

This antibody recognizes the extracellular domain of L1CAM in addition antibody recognizes the full length form (~220 kDa) and the cleaved form (~140 kDa) of L1CAM.

免疫原

Epitope: Extracellular domain
GST-tagged recombinant protein corresponding to the extracellular domain of human L1CAM.

应用

Research Category
Cell Structure
Western Blotting Analysis: 0.4 ?g/mL from a representative lot detected L1CAM in 10 ?g of rat brain tissue lysate.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected L1CAM in human kidney and human cerebellum tissue.
Anti-L1CAM Antibody is a highly specific rabbit polyclonal antibody, that targets Neural cell adhesion molecule L1 & has been tested in western blotting & IHC (Paraffin).
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.4 ?g/mL of this antibody detected L1CAM in 10 ?g of human brain tissue lysate.

目标描述

~220 / 140 kDa observed. The full length protein and the cleaved form of L1CAM have been observed at ~220 kDa and ~140 kDa, respectively (Kalus, I., et al. (2003). The Journal of Biological Chemistry. 278(12):10381-10388). Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_076493
UniProt登记号	P32004
运输	wet ice
Gene Information	human ... L1CAM(3897)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable