Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2

半岛bd体育手机客户端 说明

一般描述

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

特异性

Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.
Other species have not been tested.

免疫原

Epitope: Near cross region
Laminin isolated from EHS mouse sarcoma.

应用

Research Category
Cell Structure
Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.
Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
Research Sub Category
ECM Proteins

质量

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

目标描述

~200 kDa

外形

Format: Purified
Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
Protein G Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	AL-2, monoclonal
species reactivity	mouse
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunofluorescence: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NM_005559.2
UniProt登记号	P25391 P55268
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable