Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2

半岛bd体育手机客户端 说明

一般描述

Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.

特异性

Reacts with the heavy chain of kinesin near the N-terminus.

免疫原

Epitope: a.a.420-445
Bovine brain kinesin.

应用

Research Category
Cell Structure
Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.

EIA/RIA:
A previous lot of this antibody was used in RIA.

Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Cytoskeleton
Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.

质量

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.

目标描述

124 kDa

外形

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

储存及稳定性

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

分析说明

Control
Brain tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	H2, monoclonal
species reactivity	human, chicken, rat, sea urchin, squid, mouse
species reactivity (predicted by homology)	mammals
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable radioimmunoassay: suitable western blot: suitable
同位素/亚型	IgG2b
NCBI登记号	NP_004512
UniProt登记号	P33176
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable