Anti-Keratin Epithelial Antibody, clone AE3

半岛bd体育手机客户端 说明

特异性

Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development. AE3 and AE1 (Catalog Number MAB1612) have been used extensively for distinguishing carcinoma from non-carcinoma.

免疫原

Human epidermal cells

应用

Research Category
Cell Structure
Anti-Keratin Epithelial Antibody, clone AE3 is an antibody against Keratin Epithelial for use in WB, IH(P).
Immunohistochemistry on unfixed frozen sections or formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded sections, antigen retrieval (HEIR or trypsin treatment) is strongly recommended.

Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.

Optimal working dilutions must be determined by end user.
Research Sub Category
Cytokeratins

联系

Replaces: 04-588

外形

Format: Purified
Liquid in PBS. Contains 0.1% sodium azide.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
克隆	AE3, monoclonal
species reactivity	rabbit, bovine, monkey, mouse, human, rat, chicken
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_002273.2
UniProt登记号	P05787
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable