半岛bd体育手机客户端 说明
一般描述
Lysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle or developmental stage. KDM4A demethylates H3K36me3, a modification enriched in the 3′ end of active genes. KDM4A is also known as Lysine-specific demethylase 4A or JmjC domain-containing histone demethylation protein 3A, or Jumonji domain-containing protein 2A, and is encoded by the human gene named KDM4A/JHDM3A/JMJD2/JMJD2A/KIAA0677. KDM4A is the histone demethylase that specifically demethylates ′Lys-9′ and ′Lys-36′ residues of histone H3, thereby playing a central role in histone gene repression, and KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated forms. KDM4A does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′ nor H4 ′Lys-20′, but KDM4A does demethylate trimethylated H3 ′Lys-9′ and H3 ′Lys-36′ residues, while it has no activity on mono- and dimethylated residues. KDM4A also plays a critical role in muscle differentiation as demethylation of various targets allows influences the expression of the Myog gene family critical in muscle cell development. KDMA4A also interacts with histone deacetylases HDAC1, HDAC2, and HCAC3. It is localized in the nucleus and widely expressed. KDM4A’s own levels are mediated via ubiquitination and proteasome degradation. EMD-Millipore’s Anti-KDM4A mouse monoclonal antibody has been tested in western blot on HEK293 and KDM4A transfected HEK293 cell lysates and in paraffin embedded immunohistochemistry on human colon and larynx cancer tissues and in fluorescent immunocytochemistry on NTERA-2 cells in culture.
免疫原
Purified recombinant fragment of human KDM4A expressed in E. Coli.
应用
Anti-KDM4A Antibody, clone5H1 is a highly specific mouse monoclonal antibody, that targets Jumonji domain-containing protein (JMJD) & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KDM4A in human colon cancer and larynx cancer tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected KDM4A in NTERA-2 cells.
Optimal working dilutions must be determined by end user.
质量
Evaluated by Western Blotting in HEK293 and KDM4A-hIgGFc transfected HEK293 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected KDM4A-hIgGFc transfected HEK293 cell lysates.
目标描述
~72 kDa observed
外形
Unpurified
分析说明
Control
HEK293 and KDM4A-hIgGFc transfected HEK293 cell lysates
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 5H1, monoclonal |
species reactivity | human |
technique(s) | immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1 |
UniProt登记号 | O75164 |
运输 | wet ice |
Gene Information | human ... KDM4A(9682) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich