Anti-ISL1 Antibody, clone 1H9

半岛bd体育手机客户端 说明

一般描述

Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.

免疫原

Purified recombinant fragment of human ISL1 expressed in E. Coli. corresponding to human ISL1.

应用

Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
Detect ISL1 using this mouse monoclonal antibody, Anti-ISL1 Antibody, clone 1H9 validated for use in western blotting, Immunofluorescence & IHC.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in paraffin-embedded human lung cancer and cervical carcinoma tissues.

Optimal working dilutions must be determined by end user.

质量

Evaluated by Western Blotting in full-length ISL1-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cell lysate.

目标描述

~93 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Full-length ISL1-hIgGFc transfected HEK293 cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	1H9, monoclonal
species reactivity	human
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
UniProt登记号	P61371
运输	wet ice
Gene Information	human ... ISL1(3670)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable