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位置: 首页> 品牌> Sigma-Aldrich> Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

品牌
Sigma-Aldrich
货号
05-784R
规格纯度
clone 58-10C-31, from rabbit
价格
5016.79 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
insulin receptor substrate 1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.

特异性

Antibody recognizes the C-terminus of IRS1.

免疫原

Epitope: C-terminus
KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.

应用

Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.
Research Category
Metabolism
Research Sub Category
Insulin/Energy Signaling
Western Blot (SNAP ID) Analysis: 0.1 ?g/mL from a previous lot detected IRS-1 on 10 ?g of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.

Immunoprecipitation Analysis: 1 ?g from a previous lot immunoprecipitated IRS-1 from 100 ?g of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.

质量

Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected IRS-1 on 10 ?g of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.

目标描述

~ 160 kDa

外形

Format: Purified
Protein A purified
Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析说明

Control
3T3/A31, 3T3/L1, L6, or MCF7 cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 58-10C-31, monoclonal
species reactivity human, mouse, rat
technique(s) immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_0371
UniProt登记号 P35570
运输 dry ice
Gene Information human ... IRS1(3667)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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