Anti-IRE1p Antibody

半岛bd体育手机客户端 说明

一般描述

Inositol-requiring protein 1 (IRE1p), also known as Ire1-alpha (IRE1a), Endoplasmic reticulum-to-nucleus signaling 1 (ERN1), and Serine/threonine-protein kinase/endoribonuclease IRE1, contains 1 KEN domain and 1 protein kinase domain.
IRE1p has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1p is ubiquitously expressed with high levels observed in pancreatic tissue.

特异性

This antibody recognizes the C-terminus of IRE1p.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human IRE1p.
Epitope: C-terminus

应用

Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected IRE1p in human pancreas and kidney tissues.

Immunocytochemistry Analysis: 1 ?g/mL from a representative lot detected IRE1p in A20 cells.

Immunofluorescence Analysis: 2 ?g/mL from a representative lot detected IRE1p in A20 cells.
Detect Inositol-requiring protein 1 using this rabbit polyclonal antibody, Anti-IRE1p Antibody validated for use in western blotting, ICC & IHC.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

质量

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 2 ?g/mL of this antibody detected IRE1p in A20 cell lysate.

目标描述

~114 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Antigen Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
A20 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
UniProt登记号	O75460
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable