Anti-Inversin Antibody

半岛bd体育手机客户端 说明

一般描述

Inversin is a tubulin-associated protein, which is highly expressed in the cilia of renal tubules. It contains sixteen ankyrin repeats at the N-terminus and two IQ domains at the C-terminus. The ankyrin repeats facilitate protein-protein interactions, whereas the IQ domains are involved in calmodulin binding. Previous studies have indicated that inversin modulates the Wnt signaling pathway, and interacts with a plethora of intracellular signaling targets, including Disheveled, Frizzled-8, alpha- and beta catenins, NPHP1, and NPHP3. Inversin is localized to mitotic spindle fibers, and plays an important role in renal development and left-right axis determination. Defects in inversin result in nephronophthisis type 2, an autosomal recessive cystic kidney disease.

特异性

No homology to Rat was found.

免疫原

KLH-conjugated linear peptide corresponding to human Inversin.

应用

Research Category
Cell Structure
Anti-Inversin Antibody detects level of Inversin & has been published & validated for use in Western Blotting & IHC.
Western Blot Analysis: 1 ?g/mL from a representative lot detected Inversin in 10 ?g of human liver tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Inversin in human kidney tissue.
Research Sub Category
Cytoskeleton

质量

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected Inversin in 10 ?g of HEK293 cell lysate.

目标描述

~165/12 kDa observed. Uniprot describes 3 isoforms at ~11, ~100, and ~118 kDa The calculated molecular weights are 11 and 118 kDa, however Inversin has been shown as a ~12 and ~165 kDa band in western blots (Nurnberger, J., et al. (2004). J Am Soc Nephrol. 15:1700–1710.). Uncharacterized bands may appear at ~45, ~53, and ~55 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HEK293 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_055240
UniProt登记号	Q9Y283
运输	wet ice
Gene Information	human ... INVS(27130)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable