半岛bd体育手机客户端 说明
一般描述
Interferon Lambda 4, also known as IFN-lambda-4, and encoded by the gene IFNL4, is an important cytokine that forms part of our innate immunity against viruses. Interferon lambda-4 activates the JAK-STAT signaling pathway and ultimately up regulates several interferon specific stimulated genes. Interferon lambda-4 expression can be stimulated by nucleotide strands particularly ployinosinic-polycytidylic acid (polyI:C) which mimics the double-stranded hepatitis C virus. A genetic variation in the Interferon lambda-4 gene is associated with susceptibility to HCV infection. The mutation of a single base insertion results in gene inactivation resulting in defective immune response to HCV. Interferon lambda-4 signaling operates through Interferon lambda R1 and IL-10R2 receptors and the mutation is associated with defective glycosylation which is required for secretion, thus the amplification of the signal becomes compromised resulting in susceptibility to HCV as well as coronaviruses.
特异性
Clone 4G1 detected IFNL4 expression induction in primary hepatocytes (e.g. by PolyIC or by HCV) and exogenously overexpressed IFNL4 in HepG2 cells. IFNL4 expression is not detectable in normal liver cells (Dr. Ludmila Prokunina-Olsson, National Cancer Institute, NIH, Bethesda, MD). Clone 4G1 does not cross-react with IFN-alpha or IL-28B (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).
免疫原
Linear peptide corresponding to an internal sequence from the N-termninal half of human interferon lambda 4.
应用
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Interferon Lambda 4 in human and rat liver tissue sections.
ELISA Analysis: A representative lot was conjugated with a sulfo-tag (MSD) and employed as the detection antibody in a sandwich ELISA application. An exogenously expressed IFNL4 HaloTag
Flow Cytometry Analysis: A representative lot immunostained HepG2 cells transfected to express HaloTag
Immunocytochemistry Analysis: Representative lots immunostained HepG2 cells transfected to express HaloTag
Immunocytochemistry Analysis: A representative lot detected a time-dependent induction of IFNL4 expression in hepatocytes from a donor heterozygous for ss469415590 (TT/ΔG) upon PolyI:C treatment or hepatitis C virus (HCV) strain JFH1HCV infection in cultures (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).
Western Blotting Analysis: A representative lot detected HaloTag
Western Blotting Analysis: A representative lot detected exogenously expressed IFNL4 HaloTag
This Anti-Interferon Lambda 4 antibody, clone 4G1 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of Interferon Lambda 4.
质量
Evaluated by Western Blotting of human IFNL4 recombinant protein.
Western Blotting Analysis: 1.0 ?g/mL of this antibody detected 0.1 ?g of human IFNL4 recombinant protein.
目标描述
~21 kDa observed.
外形
Protein G Purified
Format: Purified
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 4G1, monoclonal |
species reactivity | nonhuman primates, human |
should not react with | canine, bat, giant panda, bovine, porcine |
浓度 | 1 mg/mL |
technique(s) | ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_001263183 |
UniProt登记号 | K9M1U5 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich