Anti-Integrin β4 Antibody, clone ASC-8

半岛bd体育手机客户端 说明

特异性

Human beta4 integrin (CD104)

免疫原

Human squamous cell carcinoma cells (SCC9)

应用

Research Category
Cell Structure
Research Sub Category
Integrins
Anti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.
Immunoprecipitation:A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.

Immunohistochemistry:A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).

Flow Cytometry:A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Functional Activity Assay:A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).

Working dilutions must be determined by end user.

质量

Flow Cytometry Analysis:
0.5 ?g of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 ?g of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.

联系

Replaces: 05-283

外形

Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	ASC-8, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable
同位素/亚型	IgG1κ
NCBI登记号	NM_000213.3 NM_001005619.1 NM_001005731.1
UniProt登记号	P16144
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable